Fig. 2

Multiple alignment of NS5 sequences. NS5 sequences from mosquito -borne flaviviruses, tick -borne flaviviruses, NKV flaviviruses, and some dual-host affiliated ISFs, classical ISFs, and other unclassified flaviviruses, were aligned using Clustal W. The site on which the primer design was based is also shown. The numbering of the nucleotides on the 5′ and 3′ sides corresponds to that of DENV 1 GZ/33206/D1/2014 (accession KT827377). Dots indicate identicality with the consensus sequence used for alignment. The colors indicate an individual base: “A”, yellow; “T”, green; “G”, magenta, and “C”, olive for the nucleotides in the NS5 sequences of flaviviruses; “V,” yellow; “D”, red; “R”, blue; “N”, white; “Y”, bright green for the nucleotides in the primer sequences; and “R”, blue for the nucleotides in the NS5 sequences of the Nounane virus EU159426. The names in red are reference strains, the sequences of which were used as PCR templates in this study