Fig. 7

The inhibition of EV-A71 infection by aptamer V11 and V21 in RD cells in vitro. EV-A71 was firstly pretreated by PBS buffer, random ssDNA library, aptamer V11 and V21 respectively at room temperature for 30 min. Then RD cells were infected with treated EV-A71 through incubation at 37 ℃ for 60 min. After renewal of the culture solution to remove free virion and aptamer, the RD cells were cultured at 37 ℃. a The CPE of RD cells were observed under optical microscope at 72 h post-infection. The normal untreated RD cells were adopted as the mock group. b RT-qPCR analysis was performed to detect EV-A71 mRNA in RD cells infected by original EV-A71 (PBS), random ssDNA library-medicated EV-A71 or aptamer-medicated EV-A71 at 24 h post-infection. c The expression of EV-A71 VP1 protein in RD cells infected by EV-A71 with different treatment at 48 h post-infection. The β-actin protein was employed as the internal reference protein. Data were obtained from three separate experiments and are presented as the mean ± SD. *Means P < 0.05; **means P < 0.001