Fig. 2

SARS-CoV-2 Spike glycoprotein domain structure and Spike interaction with ACE2. a Domain structure of the SARS-CoV-2 Spike comprises two subunits, S1 and S2. S1 consists of the NTD, RBD domains and the RBM within the RBD. Two cleavage sites, S1/S2’ and S2, are needed for priming and S activation for fusion of S2 and the cellular membrane to occur. S1/S2’ is cleaved by furin and S2’ by TMPRSS2 at the indicated cleavage sites. S2 subunit consists of FP; HR1 and HR2, TM, and the cytoplasmic tail (CT). b The interaction of Spike and the ACE2 receptor is defined by binding of S1 RBD and ectodomain motifs of ACE2. Specifically, the RBM of S1 RBD engage residues mainly from the α1 and α2 and β3/β4 binding motifs. (Figure created using Biorender)