Fig. 2

Rescue of recombinant ZIKA–EGFP. a Detection of EGFP by electrophoresis. (1–5 lanes are generation p1-p5 of recombinant ZIKA-EGFP, respectively) b Fluorescence of Vero cells infected with the ZIKA–EGFP (generation P5) for 48 h (×200). c Western blot detection of E gene, NS1 gene, and EGFP gene levels in Vero cells infected with the ZIKA-WT, ZIKAi (generation P5), and ZIKA–EGFP (generation P5) for 48 h. Western blot analysis was repeated three times and representative result was exhibited. d Fluorescence of C6/36 cells infected with ZIKA–EGFP at different time points. e. The localization of EGFP and E protein for ZIKA-EGFP in Vero cells (×200)