Fig. 2

A Schematic representation Luciferase constructs for X gene of EMDV. X gene constructs contain 8 and 140 nt from 5′ X gene and 3′ UTR of X gene in 5′ and 3′ sides of the luciferase gene. B Luciferase activity in luciferase reporter transcript containing the 5′ and 3′ fragments from X gene. C In vitro translation of EMDV X gene in wheat germ extract with different amount of RNA. Lane 1, 2 μl and lane 2, 0.5 μl of RNA. Lane 3, control without any RNA Lane 4, PEMV’s RNA as control