Fig. 1
Determination of the effects lithium on TNF-α production after inoculation with the RVFV. In order to measure effects of lithium in TNF-α, IL-6, IL-10, IFN-γ and RANTES production, Raw 264.7 cells were seeded at the 2.4 × 106 cells per T 25 flask for 3 h inoculated with 1 × 104.8 viral titre/mL for an hour. The excess virus was washed and the cells were treated with various concentration of lithium and then the supernatant was collected in 3, 6, 12 and 24 h time intervals. The Sandwich Elisa (PeproTech, USA) was executed to measure the amount of these cytokines from various time points. The data points represent the mean + standard deviation (error bar). The plot was developed with Graph-Pad Prism-6 software and GraphPad InStat-3 was used to establish the statistical analysis