Table 1 Overview of tests used in this study

Patient sample volume used in this study

100 μl^a

100 μl

100 μl

N/A

300 μl

Elution volume

32 μl

32 μl

32 μl

N/A

100 μl

Target gene

Orf1ab

N (CDC N1 & N2)

N/A

S and N gene

E, N and RdRp

SARS-CoV-2 specific

Yes

Yes

N/A

Yes

Yes: N & RdRp

No: E gene

Human control gene

Actin

RNase P

N/A

RNase P

None, uses PCR internal control

Criteria for positivity

CoV-2 Ct < 37

Human Ct < 35

Ct < 40

N/A

Ct < 40 with Tm matching positive control

Ct < 40