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Fig. 5 | Virology Journal

Fig. 5

From: Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

Fig. 5

Specificity analysis of TAS-ELISA using PAb PK and MAb 29B3. The specificity of the developed TAS-ELISA was investigated against the recombinant SLCMV-CP and ICMV-CP protein, partially purified SLCMV, a commercially available SLCMV positive control, and sap extracts from plants infected with SLCMV as well as other begomoviruses. The OD405 values indicate the mean values obtained from duplicate wells. Different letters on each column indicate a significant difference compared to other column at p ≤ 0.01 based on Tukey’s HSD test (SPSS software version 11.5 for Window®). The cut-off value was twice the mean OD405 of the negative controls (extraction buffer for purified recombinant CP and partially purified virus; sap extract of healthy plants for virus-infected plants)

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Virology Journal

ISSN: 1743-422X