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Fig. 3 | Virology Journal

Fig. 3

From: Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

Fig. 3

Analysis of MAb 29B3 reactivity by PTA-ELISA. Reactivity of MAb 29B3 was analyzed against recombinant SLCMV-CP, partially purified SLCMV and SLCMV-infected cassava by PTA-ELISA. The OD405 values indicate the mean values obtained from duplicate wells. Different letters on each column indicate a significant difference compared to other column at p ≤ 0.01 based on Tukey’s HSD test (SPSS software version 11.5 for Window®). The cut-off value was twice the mean OD405 of the negative controls (Coating buffer for purified recombinant SLCMV-CP and partially purified virus; sap extract of healthy cassava for SLCMV-infected cassava)

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