From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
SARS-CoV-2 | Inhibition control | Nucleic acid control | Result | Comments | ||
---|---|---|---|---|---|---|
# | E-Gene | orf1ab | KoMa | c-myc | ||
1 | < 38 | < 38 | Any | Any | Positive | |
2 | < 38* | ≤ 45* | 32 ± 3 | Signal | Positive | |
3 | ≤ 45* | < 38* | 32 ± 3 | Signal | Positive | |
4 | ≥ 38 | ≥ 38 | 32 ± 3 | Any | Ambiguous | if curves are distinctly positive; if not, analyze follow-up sample |
5 | Signal | Negative | 32 ± 3 | Signal | repeat PCR with 10 µl | |
6 | Negative | Signal | 32 ± 3 | Signal | repeat PCR with 10 µl | |
7 | Negative | Negative | 32 ± 3 | Negative | Invalid | analyze follow-up sample |
8 | Negative | Negative | > 35 | Any | Invalid | repeat extraction with 1:10 dilution |
9 | Negative | Negative | 32 ± 3 | Signal | Negative |