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Table 5 Interpretation of results obtained with the RKI/ZBS1 SARS-CoV-2 protocol

From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2

  SARS-CoV-2 Inhibition control Nucleic acid control Result Comments
# E-Gene orf1ab KoMa c-myc   
1  < 38  < 38 Any Any Positive  
2  < 38*  ≤ 45* 32 ± 3 Signal Positive  
3  ≤ 45*  < 38* 32 ± 3 Signal Positive  
4  ≥ 38  ≥ 38 32 ± 3 Any Ambiguous if curves are distinctly positive; if not, analyze follow-up sample
5 Signal Negative 32 ± 3 Signal   repeat PCR with 10 µl
6 Negative Signal 32 ± 3 Signal   repeat PCR with 10 µl
7 Negative Negative 32 ± 3 Negative Invalid analyze follow-up sample
8 Negative Negative  > 35 Any Invalid repeat extraction with 1:10 dilution
9 Negative Negative 32 ± 3 Signal Negative  
  1. Besides clearly positive and clearly negative results showing the controls as expected, unequivocal results can be obtained in particular with low concentrations of viral RNA. Measures for confirmation of such results are described in the table
  2. *If ΔCT value is > 3 repeat PCR with 10 µl of RNA template