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Table 3 Master mix PCR reaction 1 E-Gene/KoMa

From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2

Reagent Vol. (µL)
Water 2.25
2× RT-PCR buffer 12.50
nCoV E_Sarbeco F1 (10 µM) 1.00
nCoV E_Sarbeco R2 (10 µM) 1.00
E_Sarbeco P1 FAM (10 µM) 0.50
KoMa F (10 µM) 0.75
KoMa R (10 µM) 0.75
KoMa TM TexRed (10 µM) 0.25
25× RT-PCR Enzyme mix 1.00
Mix vol 20.00
RNA vol 5.00
Total vol 25.00
  1. Volumes used for reaction set up, concentrations of stock solutions are given in brackets