Table 3 Master mix PCR reaction 1 E-Gene/KoMa
From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
Reagent | Vol. (µL) |
|---|---|
Water | 2.25 |
2× RT-PCR buffer | 12.50 |
nCoV E_Sarbeco F1 (10 µM) | 1.00 |
nCoV E_Sarbeco R2 (10 µM) | 1.00 |
E_Sarbeco P1 FAM (10 µM) | 0.50 |
KoMa F (10 µM) | 0.75 |
KoMa R (10 µM) | 0.75 |
KoMa TM TexRed (10 µM) | 0.25 |
25× RT-PCR Enzyme mix | 1.00 |
Mix vol | 20.00 |
RNA vol | 5.00 |
Total vol | 25.00 |