Impact of the N501Y substitution of SARS-CoV-2 Spike on neutralizing monoclonal antibodies targeting diverse epitopes

Table 1 Neutralizing activity and binding kinetics of nAbs against the wild type and N501Y mutant SARS-CoV-2

Antibody	Group^a	Contact with N501	Structure	References	Neutralization (IC₅₀, µg/ml, mean)^b			Affinity (K_D, nM, mean)^c
Antibody	Group^a	Contact with N501	Structure	References	WT	N501Y	FC^d	WT	N501Y	FC^d
P2C-1F11	Class 1	No	PDB: 7CDI	Ge et al. [13]	0.0300	0.0180	0.60	0.526	0.241	0.46
REGN10933	Class 1	No	PDB: 6XDG	Hansen, et al.[3]	0.0015	0.0035	2.36	0.0645	0.123	1.91
CB6	Class 1	Yes	PDB: 7C01	Shi et al. [1]	0.0456	0.3363	7.38	0.321	2.14	6.67
BD-23	Class 2	Yes	PDB: 7BYR	Cao et al. [21]	16.3052	2.4771	0.15	137	20.6	0.15
P2B-2F6	Class 2	No	PDB: 7BWJ	Ju et al. [2]	0.1644	0.0697	0.42	0.667	0.267	0.40
P2C-1A3	Class 2	No	PDB: 7CDJ	Ge et al. [13]	0.1862	0.2833	1.52	0.247	0.167	0.68
BD-368–2	Class 2	No	PDB: 7CHH	Du et al. [15]	0.0004	0.0008	2.11	0.0649	0.0368	0.57
S309	Class 3	No	PDB: 6WPS	Pinto et al. [17]	0.1641	0.4307	2.62	< 0.001	< 0.001	n.a.^e
REGN10987	Class 3	No	PDB: 6XDG	Hansen, et al.[3]	0.0062	0.0039	0.63	0.108	0.0587	0.54
EY6A	Class 4	No	PDB: 6ZDH	Zhou et al. [16]	7.0680	10.7957	1.53	0.111	0.134	1.21
COV2-2196	n.a	n.a	n.a	Zost et al. [4]	0.0032	0.0029	0.93	0.0681	0.0581	0.85
CV07-287	n.a	n.a	n.a	Kreye et al. [5]	0.2930	0.2610	0.89	1.19	1.25	1.05

^aAntibodies were classified into four groups based on their competitions with ACE2 and recognitions with ‘up’/‘down’ conformations of RBD [12]
^bNeutralization was measured as IC₅₀ in µg/ml of nAbs against SARS-CoV-2 pseudovirus
^cAffinity was measured as K_D in nM of nAbs binding to SARS-CoV-2 RBD by SPR
^dFC, Fold change. N501Y/WT
^en.a., not available

ISSN: 1743-422X