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Table 1 Neutralizing activity and binding kinetics of nAbs against the wild type and N501Y mutant SARS-CoV-2

From: Impact of the N501Y substitution of SARS-CoV-2 Spike on neutralizing monoclonal antibodies targeting diverse epitopes

Antibody Groupa Contact with N501 Structure References Neutralization (IC50, µg/ml, mean)b Affinity (KD, nM, mean)c
WT N501Y FCd WT N501Y FCd
P2C-1F11 Class 1 No PDB: 7CDI Ge et al. [13] 0.0300 0.0180 0.60 0.526 0.241 0.46
REGN10933 Class 1 No PDB: 6XDG Hansen, et al.[3] 0.0015 0.0035 2.36 0.0645 0.123 1.91
CB6 Class 1 Yes PDB: 7C01 Shi et al. [1] 0.0456 0.3363 7.38 0.321 2.14 6.67
BD-23 Class 2 Yes PDB: 7BYR Cao et al. [21] 16.3052 2.4771 0.15 137 20.6 0.15
P2B-2F6 Class 2 No PDB: 7BWJ Ju et al. [2] 0.1644 0.0697 0.42 0.667 0.267 0.40
P2C-1A3 Class 2 No PDB: 7CDJ Ge et al. [13] 0.1862 0.2833 1.52 0.247 0.167 0.68
BD-368–2 Class 2 No PDB: 7CHH Du et al. [15] 0.0004 0.0008 2.11 0.0649 0.0368 0.57
S309 Class 3 No PDB: 6WPS Pinto et al. [17] 0.1641 0.4307 2.62  < 0.001  < 0.001 n.a.e
REGN10987 Class 3 No PDB: 6XDG Hansen, et al.[3] 0.0062 0.0039 0.63 0.108 0.0587 0.54
EY6A Class 4 No PDB: 6ZDH Zhou et al. [16] 7.0680 10.7957 1.53 0.111 0.134 1.21
COV2-2196 n.a n.a n.a Zost et al. [4] 0.0032 0.0029 0.93 0.0681 0.0581 0.85
CV07-287 n.a n.a n.a Kreye et al. [5] 0.2930 0.2610 0.89 1.19 1.25 1.05
  1. aAntibodies were classified into four groups based on their competitions with ACE2 and recognitions with ‘up’/‘down’ conformations of RBD [12]
  2. bNeutralization was measured as IC50 in µg/ml of nAbs against SARS-CoV-2 pseudovirus
  3. cAffinity was measured as KD in nM of nAbs binding to SARS-CoV-2 RBD by SPR
  4. dFC, Fold change. N501Y/WT
  5. en.a., not available
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Virology Journal

ISSN: 1743-422X