Table 2 Oligonucleotide primers and TaqMan probes used for specific detection of PCV2 genotypes
From: Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR
Primer/probe | Sequence 5′–3′ | Length |
|---|---|---|
PCV2a_V1-F | ATC AAT AGT GGA RTC RAG AAC AG | 23 |
PCV2a_V2-F | ATC AAT RGT GGA ATC AAG GAC | 21 |
PCV2a-R | CGG TGG ACA TGM TGA GAT | 18 |
PCV2b-F | TCA ATA GTG GAA TCT AGG ACA GG | 23 |
PCV2b-R | CGG TKG ACA TGM TGA GAT T | 19 |
PCV2c-R | GTT AAT GCC TCA CAG GTC T | 19 |
PCV2d-F | AAT CGA TTG TCC YAT CAA G | 19 |
PCV2d-R | AAC GCC CTC CTG GAA T | 16 |
PCV2e-F | AAT CGA TTA TCC TAT CAA GGA CA | 23 |
PCV2e-R | TTG GAG ATT TCC TCC CAC | 18 |
PCV2f-F | TTT CTC ACT TTG TGT TAA GAG | 21 |
PCV2f-R | GTA AAT ACG ACC AGG ACT ACA | 21 |
PCV2g_V1-F | CGA TTG TCC TGT CAA GGA C | 19 |
PCV2g_V2-F | ATC GAT TGT CCT ATC AAG GA | 20 |
PCV2h_V1-F | AAT CAA TAG TGG AAT CAA GAA CAG | 24 |
PCV2h_V2-F | ATC GAT AGT GGA ATC AAG AAC AG | 23 |
Probe PCV2a | FAM- GGT ATA GAG ATT TTG TTG GTC C -BHQ1 | 22 |
Probe PCV2b | FAM- ACA GAG CGG GGG TTT GA -BHQ1 | 17 |
Probe PCV2cgh | FAM- CAC AGT GAG GGG GTT TGA G -BHQ1 | 19 |
Probe PCV2d | FAM- ACA GTG AGG GGG TTT GA -BHQ1 | 17 |
Probe PCV2e | FAM- GGG TAC AGA GAG GGG GTT TGT T -BHQ1 | 22 |
Probe PCV2f | FAM- CTA AAT TGT ACA TAA ACG GTT ATA C -BHQ1 | 25 |