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Table 2 Oligonucleotide primers and TaqMan probes used for specific detection of PCV2 genotypes

From: Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR

Primer/probe Sequence 5′–3′ Length
PCV2a_V1-F ATC AAT AGT GGA RTC RAG AAC AG 23
PCV2a_V2-F ATC AAT RGT GGA ATC AAG GAC 21
PCV2a-R CGG TGG ACA TGM TGA GAT 18
PCV2b-F TCA ATA GTG GAA TCT AGG ACA GG 23
PCV2b-R CGG TKG ACA TGM TGA GAT T 19
PCV2c-R GTT AAT GCC TCA CAG GTC T 19
PCV2d-F AAT CGA TTG TCC YAT CAA G 19
PCV2d-R AAC GCC CTC CTG GAA T 16
PCV2e-F AAT CGA TTA TCC TAT CAA GGA CA 23
PCV2e-R TTG GAG ATT TCC TCC CAC 18
PCV2f-F TTT CTC ACT TTG TGT TAA GAG 21
PCV2f-R GTA AAT ACG ACC AGG ACT ACA 21
PCV2g_V1-F CGA TTG TCC TGT CAA GGA C 19
PCV2g_V2-F ATC GAT TGT CCT ATC AAG GA 20
PCV2h_V1-F AAT CAA TAG TGG AAT CAA GAA CAG 24
PCV2h_V2-F ATC GAT AGT GGA ATC AAG AAC AG 23
Probe PCV2a FAM- GGT ATA GAG ATT TTG TTG GTC C -BHQ1 22
Probe PCV2b FAM- ACA GAG CGG GGG TTT GA -BHQ1 17
Probe PCV2cgh FAM- CAC AGT GAG GGG GTT TGA G -BHQ1 19
Probe PCV2d FAM- ACA GTG AGG GGG TTT GA -BHQ1 17
Probe PCV2e FAM- GGG TAC AGA GAG GGG GTT TGT T -BHQ1 22
Probe PCV2f FAM- CTA AAT TGT ACA TAA ACG GTT ATA C -BHQ1 25
  1. Bold and underlined: nucleotide with LNA modification