Table 1 The primers of HPV39 E6/E7/L1
From: Genetic variability of human papillomavirus type 39 based on E6, E7 and L1 genes in Southwest China
Gene | Primer sequences | Primer location (nt) | Amplification length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
E6 | F: 5′-GGGAGTAACCGAAAACGGTC-3’ | 36–55 | 821 | 57 |
R: 5′-GTTGTCGCAGAGTATCCCGT-3’ | 856–837 | |||
E7 | F: 5′-GGACCGCAGACTAACACGAA-3’ | 187–206 | 613 | 57 |
R: 5′-CCCTTTGGGCCTCTTGCATA-3’ | 799–780 | |||
L1-1 | F: 5′-GCTCACTACCTTCTGTGGCTT-3’ | 105–125 | 1059 | 57 |
R: 5′-ACCCACCATACCACCACGAT-3’ | 1163–1144 | |||
L1-2 | F: 5′-ATTGGGGAGCACTGGGGTAA-3’ | 72–91 | 1332 | 56 |
R: 5′-ACTTCGGTCGCCACAAAATG-3’ | 1403–1384 |