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Table 2 Primers used for PCR amplification and sequencing. Column 1: Gene name. Column 2: primers sequence. Column 3: PCR product length

From: Targeting the Kaposi’s sarcoma-associated herpesvirus genome with the CRISPR-Cas9 platform in latently infected cells

Gene

Primer pair

*Size of PCR product

gfp

1st F: TCTTCTTCAAGGACGACGGC

2nd F: GCAGCTCGCCGACCACTAC

1st + 2nd R: TTACTTGTACAGCTCGTCCA

462 bp

226 bp

orf73

1st F: AGATGTGACCTTGGCGATG

2nd F: CAACCGCGAAGGAAGCAT

1st + 2nd R: CGGAGACACAGGATGGGATG

313 bp

282 bp

  1. *Sizes ignore potential deletions and insertions due to single or double cut by Cas9
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Virology Journal

ISSN: 1743-422X