Fig. 6

Distribution and abundance of deletions flanking CRISPR-Cas9 cut sites. The graphs show the percentage of nucleotide deletion based on amplicon deep sequencing. Cutting site in orf73 (a–h), cutting site in gfp hygromycin minus (i–l) and no guide was used (a, b, i, j) (control). (a, c, e, g, i, k) the graphs show the most abundant deletion length. (b, d, f, h, j, l) the graph shows the distribution of deletions around the cutting site (0). Time points are indicated in the title. a, c, e, g, i, k the x-axis represent the length of the most highly frequent deletions. b, d, f, h, j, l the x-axis represent the frequency of a base-pair to be deleted, in both sides of the cutting cite (0- between nucleotides 3 and 4 5′ to the pam sequence