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Table 1 Inhibition of bacteria and yeasts by VTM

From: Optimal preparation of SARS-CoV-2 viral transport medium for culture

Organism Inoculum Filtered Autoclaved γ-irradiated
Streptococcus pyogenes  +++  NG NG NG
Escherichia coli  +++  NG NG NG
Staphylococcus aureus  +++  NG NG NG
Candida albicans  +++  NG  +++   +++ 
Saccharomyces cerevisiae  +++  NG  +   +++ 
  1. Stationary phase cultures of 3 bacteria and 2 yeasts were diluted and inoculated into VTM with concentrations between 103 and 104 bacteria or yeast, mixed and 10 µl was immediately removed and plated onto horse blood agar (Inoculum). The VTM was then incubated for 3 days at RT (18–20 °C) whereafter 10ul was removed and plated onto HBA. The growth on HBA was determined. No growth (NG), +  < 10 colonies, +++  > 300