Optimal preparation of SARS-CoV-2 viral transport medium for culture

Table 1 Inhibition of bacteria and yeasts by VTM

Organism	Inoculum	Filtered	Autoclaved	γ-irradiated
Streptococcus pyogenes	+++	NG	NG	NG
Escherichia coli	+++	NG	NG	NG
Staphylococcus aureus	+++	NG	NG	NG
Candida albicans	+++	NG	+++	+++
Saccharomyces cerevisiae	+++	NG	+	+++

Stationary phase cultures of 3 bacteria and 2 yeasts were diluted and inoculated into VTM with concentrations between 10³ and 10⁴ bacteria or yeast, mixed and 10 µl was immediately removed and plated onto horse blood agar (Inoculum). The VTM was then incubated for 3 days at RT (18–20 °C) whereafter 10ul was removed and plated onto HBA. The growth on HBA was determined. No growth (NG), + < 10 colonies, +++ > 300

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