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Fig. 2 | Virology Journal

Fig. 2

From: Inhibition of Japanese encephalitis virus proliferation by long non-coding RNA SUSAJ1 in PK-15 cells

Fig. 2

Effects of lncRNA-SUSAJ1 knockdown on JEV proliferation. (a) Knockdown of lncRNA-SUSAJ1 in PK-15 cells by antisense oligonucleotides (ASO). Transcript levels after transfection with lncRNA ASO for 24 h, determined by RT-PCR (n = 3 per group) * P <  0.05 and **P < 0.01 VS ASO NC. (b) Time course of lncRNA-SUSAJ1 transcript levels after transfection with lncRNA ASO1, determined by RT-PCR (n = 3 per group), *P < 0.05 VS ASO 0 h. (c) Whole-cell lysates were harvested from the treatment groups as indicated. Immunoblotting was performed to detect NS3 protein levels using β-actin as an internal control. (D) Effects of JEV transcript levels were determined by RT-PCR after transfected with ASO (lncRNA-SUSAJ1) and ASO (NC) (n = 3 per group), **P < 0.01 VS ASO (NC)

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