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Table 2 Evaluation of 49 patients (101 swabs) with discordant COVID-19 testing and confirmatory testing results

From: False negative rate of COVID-19 PCR testing: a discordant testing analysis

 

Swab 1 (n = 49 swabs)

Swab 2 (n = 49 swabs)

Swab 3 (n = 3 swabs)c

NP swab in UTM (%)

29 (59.2)

32 (65.3)

2 (66.7)

Deep nasal turbinate swab (%)

20 (40.8)

9 (18.4)

1 (33.3)

Oropharyngeal swab (%)

0

8 (16.3)

0

Original swab test resulta

(E gene Ct median; range)

(RdRp gene Ct median; range)

49 negative

46 positive

3 negative

(19.5; 13.0–35.5)

(22.4; 16.2–37.8)

3 positive

0 negative

(13.9; 12.5–24.7)

(16.5; 14.8–28.0)

Positive result on E gene assayb

(Ct range)

5/49

(34.1–37.9)

0/3

ND

Positive result on CDC N1 assayb

(Ct range)

2/49

(32.7–35.9)

0/3

ND

Positive result on CDC N2 assayb

(Ct range)

5/49

(33.2–38.8)

0/3

ND

  1. Ct cycle threshold (cycles), E envelope, LDT laboratory developed test, ND not done, NP nasopharyngeal, RdRp RNA dependent RNA polymerase, UTM universal transport media
  2. aTesting done using E gene/RdRp gene LDT SARS-CoV-2 rtRT-PCR
  3. bRepeat testing conducted only on negative swabs to evaluate for false-negative results
  4. cOnly 3 of 49 patients had a third swab done