Fig. 3

Representative CFSE staining of CD8⁺ T cell proliferation stimulated with HTNV MAP and single peptide. Isolated PBMCs from HLA-A*02⁺ patients with HFRS were labeled with CFSE (5 μM) and stimulated with HTNV MAP or single epitope LL9, VV9, SL9 and FA9. The cells were stained with anti-CD3 PerCP-Cy5.5 and anti-CD8 PE mAb after 5 days of culture. a The scatter plot for the flow cytometry analysis of cell proliferation from one representative HLA-A*02⁺ HFRS patient after stimulation with different groups of stimuli when gated on CD3⁺ cells. The upper left quadrant shows that the CFSE fluorescence intensity was reduced in cells representing the proliferation percentages. The number indicates the percentage of cells in the boxed area. b The histogram shows the peak shift of the CFSE^dimCD3⁺CD8⁺T cells in different stimulation groups. The degree of shift to the left of the curve reflects the proliferation percentage of CD8⁺ T cells. The SEB stimulation group was used as a positive control, and the no stimulation group was used as a negative control