Fig. 5

Downregulation of miR-876-5p inhibited EV-A71 replication and expression by modulating CREB5. SF268 cells were transfected with either anti-miR-876-5p-inhibitor only or in combination with si-CREB5 or si-Scramble as negative control. a SF268 cells were treated with the miRNA hairpin negative control (anti-miR-control), anti-miR-876-5p, anti-miR-876-5p and si-scramble RNA (the negative control for si-CREB5 RNA), or anti-miR-876-5p and si-CREB5 RNA for 24 h, followed by EV-A71 infection. The cells were harvested at 24 h after the EV-A71 infection, and viral RNA was examined by real-time-qPCR of each sample. b EV-A71 viral 3D protein was detected by Western blotting using anti-3D specific antibody. β-actin was detected as the loading control. c Progeny viruses were measured in culture supernatant by a plaque-forming assay. All experiments were repeated three times. * P < .05; ** P < .01