Fig. 2

miR-876-5p preferentially regulated the EV-A71 life cycle. a SF268 cells were introduced to an anti-miR-876-5p inhibitor and then cultivated for 24 h. The expression level of miR-876-5p was quantified by real-time polymerase chain reaction (RT-qPCR). The anti-miR-876-5p-inhibitor-treated cells were infected with EV-A71 (MOI = 1) for 24 h. Both the (+)strand of viral RNA (b) and (−)strand of viral RNA (c) were quantified through RT-qPCR; d protein expression levels of viral 3D/3CD and VP1–3 were detected by Western blot using anti-3D and anti-VP specific antibodies (lane 2: Negative control cells; lane 3: anti-miR-876-5p inhibitor treatment); e The infectivity of EV-A71 progeny from the anti-miR-876-5p-inhibitor-treated cells was assessed in the plaque assay. The virus titers are shown in PFU/mL. The data shown are based on the mean of three independent experiments. * P < .05; ** P < .01