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Table 4 Adsorption dynamics of bacteriophages vB_KpnS_FZ10, vB_KpnS_FZ41, vB_KpnP_FZ12, and vB_KpnM_FZ14 on Klebsiella pneumoniae cells

From: Characterization of four virulent Klebsiella pneumoniae bacteriophages, and evaluation of their potential use in complex phage preparation

Bacteriophage Bacteriophage titer, pfu/ml
Incubation time, 0 min Incubation time, 5 min % of adsorbed phages Adsorption constant, k
vB_KpnS_FZ10 \(2.8 \times 10^{7} \pm 8.2 \times 10^{6}\) \(3.2 \times 10^{6} \pm 1.6 \times 10^{5}\) \(89\) \(4.3 \times 10^{ - 9}\)
vB_KpnS_FZ41 \(1.1 \times 10^{7} \pm 1.2 \times 10^{6}\) \(2.3 \times 10^{6} \pm 0.8 \times 10^{5}\) \(79\) \(3.1 \times 10^{ - 9}\)
vB_KpnP_FZ12 \(3.9 \times 10^{7} \pm 1.4 \times 10^{6}\) \(2.7 \times 10^{6} \pm 1 \times 10^{5}\) \(93\) \(5.3 \times 10^{ - 9}\)
vB_KpnM_FZ14 \(2.3 \times 10^{7} \pm 2 \times 10^{6}\) \(3 \times 10^{6} \pm 1.6 \times 10^{5}\) \(87\) \(4 \times 10^{ - 9}\)
  1. Dependence of the titer of bacteriophages not adsorbed on Klebsiella pneumoniae cells on the incubation time in the phage-cell system is indicated. The average value (± SD) is calculated based on the results of three repetitions. The reduction in phage titer was the number of phages adsorbed on the cells. The percentage of viruses adsorbed on cells and adsorption constant are calculated