Fig.4.

3′-Processing inhibition efficiency of catalytic and non-catalytic integrase inhibitors. a Schematic diagram of the 3′-processing inhibition assay. The 3′-processing inhibition assay was performed by modifying the protocol of the strand transfer assay. The green and gray boxes represent the response under conditions of NCINI or INSTI, respectively. b For determination of 3′-processing inhibition efficiency, the strand transfer assay protocol was modified. Before addition of LTR dsDNA, fivefold serially diluted integrase inhibitors were incubated with 20 nM integrase for 30 min. The integrase-inhibitor mixtures were treated with LTR dsDNA-conjugated 96-well plates. After incubation, target DNA was added to each well for the strand transfer reaction and the products detected colorimetrically using a HRP-labeled antibody against 3′-modified target DNA. "No integrase" and "integrase-only" wells were set as the negative and positive control, respectively