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Fig. 1 | Virology Journal

Fig. 1

From: Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Fig. 1

Linear relationship of qRT-PCR, OSN-qRT-PCR and ddPCR for quantifying SARS-CoV-2 pseudoviral RNA standards for ORF1ab and N gene. a, b Expected values (converted to log10) were plotted on the X axis versus measured values of qRT-PCR (converted to log10) on the Y axis targeting a ORF1ab and b N. c, d Expected values(converted to log10) were plotted on the X axis versus measured Ct values of OSN-qRT-PCR on the Y axis targeting c ORF1ab and d N. e, f Expected values (converted to log10) were plotted on the X axis versus measured values (converted to log10) of ddPCR on the Y axis using Graph Pad Prism targeting e ORF1ab and f N

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