Skip to main content

Table 5 Summary of missense mutations on partial RdRP gene of SARS-CoV-2 and corresponding amino acid changes. Nucleotide and amino acid positions correspond to reference sequences NC_045512.2 and YP_009725307.1, respectively

From: Evaluation on the use of Nanopore sequencing for direct characterization of coronaviruses from respiratory specimens, and a study on emerging missense mutations in partial RdRP gene of SARS-CoV-2

SNVs Amino acid changes No. of sequences Remarks
15319C > T P627S 1 §End of conserved polymerase motif A (17/17), containing the classic divalent-cation-binding residue D618, related to RdRP fidelity
15327G > A M629I 2  
15327G > C M629I 1  
15327G > T M629I 13  
15328C > T L630F 2  
15338T > C M633T 1  
15346C > A L636I 2  
15346C > T L636F 4  
15349G > C V637L 1  
15352C > T L638F 1  
15356C > T A639V 2  
15358C > A R640S 1  
15359G > A R640H 1  
15364C > A H642N 1 §Zn binding site (6/8)
15368C > T T643I 4  
15371C > T T644M 4  
15380G > T S647I 47  
15384G > T L648F 1  
15392G > A R651H 1  
15406G > A A656T 17  
15406G > T A656S 1  
15407C > T A656V 1  
15412G > T E658* 1  
15418G > T A660S 3  
15438G > T M666I 34 §End of nsp8 interaction site (52/52)
15439G > A V667I 5  
15444G > T M668I 3  
15448G > A G670S 1  
15448G > T G670C 1  
15451G > A G671S 2  
15452G > T G671V 2  
15460T > C Y674H 2  
15463G > T V675F 2  
15472G > T G678C 1  
15488G > T G683V 1 §Conserved polymerase motif B (4/30), related to RdRP fidelity
15491A > G D684G 1 §Conserved polymerase motif B (5/30), related to RdRP fidelity
15535G > T A699S 3 §Conserved polymerase motif B (20/30), related to RdRP fidelity
15538G > A V700I 1 §Conserved polymerase motif B (21/30), related to RdRP fidelity
15539T > C V700A 1 §Conserved polymerase motif B (21/30), related to RdRP fidelity
15553A > G N705D 1 §Conserved polymerase motif B (26/30), related to RdRP fidelity
15571G > T D711Y 1  
15574G > A G712S 1  
15575G > T G712V 1  
15586G > T A716S 1  
15589G > T D717Y 1  
15593A > G K718R 2  
15594G > T K718N 1  
15596A > G Y719C 2  
15598G > T V720F 1  
15602G > A R721H 1  
15613C > T H725Y 2  
15619C > T L727F 1  
15627G > T E729D 1  
15636T > A Y732* 1  
15638G > A R733K 1  
15640A > T N734Y 1  
15641A > C N734T 1  
15647A > G D736G 4  
15652G > T D738Y 2  
15656C > T T739I 1  
15664G > A V742M 1  
15665T > C V742A 1  
15665T > G V742G 1  
15668A > G N743S 1  
15672G > T E744D 8  
15682T > A Y748N 1  
15683A > T Y748F 1  
15685T > A L749M 1  
15688C > T R750C 1  
15689G > A R750H 1  
15696T > G H752Q 1  
  1. §Depicted from NCBI Conserved Domain database [14]