Fig. 3
Luteolin inhibits RSV replication in vivo. BALB/c mice were intraperitoneally injected with luteolin before intranasally infected with 5 × 105 PFU RSV. At 1- or 3-days post infection, a Virus titer and RSV-F mRNA expression in lung homogenates were measured by plaque assay and RT-qPCR, respectively. b The mRNA level of MX1, OAS1 and ISG15 in lung tissues were detected by RT-qPCR. c IFN-α and IFN-β mRNA expression in lung tissues or secretion in BALF were determined by RT-qPCR or ELISA, respectively. d Lung tissues were collected for H&E staining and the representative histological changes of the lung were presented (original magnification 200 × , Scale bar: 50 μm). e BALB/c mice were intraperitoneally injected with luteolin before intranasally infected with 1 × 107 PFU RSV. At 1- or 3-days post infection, virus titer and RSV-F mRNA expression in lung homogenates were measured by plaque assay and RT-qPCR, respectively. Data shown are means ± SEM. Statistical significance was examined by Students’ t-test. P < 0.05 was considered statistically significant. *P < 0.05, **P < 0.01, ***P < 0.001. RSV respiratory syncytial virus, MX1 MX dynamin like GTPase 1, OAS1 2′-5′-oligoadenylate synthase 1, ISG15 interferon stimulated gene 15, IFN interferon, BALF bronchial alveolar lavage fluid