Table 2 Ct values and viral loads (viral RNA copies/uL of RNA extraction solution) for samples processed with "2019-nCoV CDC EUA kit" and ""AccuPower SARS-CoV-2 kit"
From: Poor sensitivity of "AccuPower SARS-CoV-2 real time RT-PCR kit (Bioneer, South Korea)"
Sample | Sample ID | 2019-nCoV CDC EUA | Accupower SARS-CoV-2 (Bioneer) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Viral Load (copies/uL) | N1 Ct | N2 Ct | RP Ct | Result CDC | E Ct | IPC (M1)Ct | RdRp Ct | IPC (M2) Ct | Result Bioneer | ||
1 | 13517 | 2.03 × 107 | 12.16 | 13.13 | 20.16 | Positive | 15.38 | 25.95 | 15.38 | 25.67 | Positive |
2 | 11957 | 1.11 × 107 | 13.08 | 14.04 | 27.02 | Positive | 16.10 | 26.31 | 16.19 | 26.14 | Positive |
3 | 13645 | 2.32 × 106 | 16.15 | 17.21 | 21.28 | Positive | 23.25 | 25.44 | 21.69 | 26.10 | Positive |
4 | 11971 | 6.10 × 105 | 17.50 | 18.78 | 23.35 | Positive | 21.31 | 26.06 | 21.29 | 25.92 | Positive |
5 | 13466 | 2.04 × 105 | 19.17 | 21.31 | 21.01 | Positive | 23.06 | 25.52 | 22.27 | 25.81 | Positive |
6 | 13468 | 1.61 × 105 | 19.53 | 22.02 | 20.23 | Positive | 23.47 | 25.94 | 23.50 | 25.99 | Positive |
7 | 12116 | 1.36 × 105 | 20.57 | 20.66 | 24.29 | Positive | 27.01 | 26.78 | 25.26 | 26.07 | Positive |
8 | 12397 | 1.20 × 105 | 21.61 | 22.25 | 21.04 | Positive | 24.28 | 25.93 | 24.11 | 24.93 | Positive |
9 | 13441 | 2.75 × 104 | 22.70 | 23.62 | 24.34 | Positive | 26.26 | 26.00 | 26.06 | 25.65 | Positive |
10 | 11944 | 1.79 × 104 | 24.02 | 24.69 | 20.54 | Positive | 27.20 | 26.09 | 27.55 | 26.03 | Positive |
11 | 13632 | 1.66 × 104 | 23.26 | 24.60 | 23.83 | Positive | 28.12 | 25.62 | 27.93 | 25.89 | Positive |
12 | 12121 | 2.12 × 103 | 26.87 | 27.39 | 19.30 | Positive | 30.23 | 26.03 | 31.12 | 25.65 | Positive |
13 | 13469 | 1.58 × 103 | 26.58 | 29.64 | 23.00 | Positive | 30.34 | 25.82 | 31.27 | 25.75 | Positive |
14 | 12079 | 1.46 × 103 | 27.23 | 28.06 | 20.15 | Positive | 29.60 | 25.99 | 30.23 | 25.74 | Positive |
15 | 12123 | 7.49 × 102 | 28.41 | 29.09 | 20.07 | Positive | 31.60 | 25.90 | 32.98 | 25.68 | Positive |
16 | 12092 | 6.24 × 102 | 28.68 | 29.24 | 19.36 | Positive | 32.06 | 26.04 | 33.23 | 25.98 | Positive |
17 | 13617 | 4.53 × 102 | 28.66 | 31.31 | 21.41 | Positive | NA | 28.98 | NA | 26.33 | Negative |
18 | 11949 | 4.34 × 102 | 28.71 | 30.48 | 22.40 | Positive | 33.08 | 26.42 | 33.85 | 26.19 | Positive |
19 | 13653 | 4.24 × 102 | 28.54 | 30.37 | 19.11 | Positive | 31.67 | 25.87 | 33.00 | 25.79 | Positive |
20 | 13523 | 3.56 × 102 | 28.96 | 31.21 | 17.39 | Positive | 31.73 | 26.17 | 32.27 | 26.03 | Positive |
21 | 13598 | 3.37 × 102 | 29.03 | 31.07 | 19.15 | Positive | 32.77 | 25.63 | 34.33 | 25.54 | Positive |
22 | 13560 | 2.97 × 102 | 29.19 | 30.64 | 19.42 | Positive | NA | 25.93 | 35.54 | 25.81 | Inconclusive |
23 | 12107 | 2.66 × 102 | 29.94 | 30.92 | 19.65 | Positive | 34.02 | 26.23 | 20.16 | 24.55 | Positive |
24 | 11967 | 2.50 × 102 | 29.39 | 32.14 | 23.86 | Positive | 32.74 | 25.81 | 34.08 | 25.79 | Positive |
25 | 9224 | 2.23 × 102 | 29.47 | 30.94 | 24.89 | Positive | 33.16 | 26.06 | 33.20 | 26.13 | Positive |
26 | 9220 | 1.97 × 102 | 29.65 | 31.07 | 27.88 | Positive | NA | 26.09 | 37.73 | 26.22 | Inconclusive |
27 | 12451 | 1.36 × 102 | 30.18 | 32.29 | 23.53 | Positive | NA | 26.08 | 35.07 | 25.66 | Inconclusive |
28 | 13489 | 1.06 × 102 | 30.49 | 32.88 | 20.17 | Positive | NA | 25.64 | 34.88 | 25.74 | Inconclusive |
29 | 13522 | 9.17 × 101 | 30.68 | 34.30 | 18.99 | Positive | NA | 26.07 | 36.57 | 25.72 | Inconclusive |
30 | 13644 | 8.10 × 101 | 30.92 | 32.81 | 22.91 | Positive | NA | 25.81 | NA | 25.70 | Negative |
31 | 13636 | 6.20 × 101 | 31.31 | 34.54 | 23.44 | Positive | NA | 26.04 | NA | 25.67 | Negative |
32 | 11966 | 5.73 × 101 | 31.63 | 33.47 | 25.19 | Positive | NA | 25.87 | 36.38 | 25.69 | Inconclusive |
33 | 11948 | 3.33 × 101 | 31.96 | 35.35 | 22.13 | Positive | NA | 26.31 | 36.61 | 26.03 | Inconclusive |
34 | 13674 | 1.92 × 101 | 32.65 | 36.06 | 21.26 | Positive | NA | 25.76 | NA | 25.61 | Negative |
35 | 13521 | 1.10 × 101 | 34.15 | 36.61 | 21.26 | Positive | NA | 26.29 | NA | 26.03 | Negative |
36 | 11946 | 6.77 | 33.97 | 38.15 | 21.97 | Positive | NA | 26.02 | NA | 25.78 | Negative |
37 | 13616 | 4.45 | 34.50 | 38.68 | 19.66 | Positive | NA | 25.99 | NA | 25.77 | Negative |
38 | 13630 | 2.87 | 34.88 | 38.58 | 23.17 | Positive | NA | 25.60 | NA | 25.49 | Negative |