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Table 4 Comparison of RT-qPCR Quantification Cycle (Cq) values to RT-LAMP positive results

From: Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Cq value RT-qPCR

Positive result RT-LAMP ORF8

Positive result RT-LAMP N

< 20

5 out of 5 (100%)

5 out of 5 (100%)

20–25

9 out of 9 (100%)

9 out of 9 (100%)

25–30

12 out of 14 (86%)

13 out of 14 (93%)

30–35

6 out of 7 (86%)

0 out of 7 (0%)

> 35

2 out of 5 (40%)

0 out of 5 (0%)

  1. 40 pharyngeal swab samples were routinely tested for COVID-19 using ampliCube Coronavirus SARS-CoV-2 kit acquired from Mikrogen Diagnostik, where Orf1a serves as specific target for SARS-CoV-2. The same pharyngeal swab samples were pre-heated at 90 °C for 5 min and directly tested for SARS-CoV-2 in RT-LAMP. The targets ORF8 and N detect SARS-CoV-2 nucleic acid in the swab samples
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Virology Journal

ISSN: 1743-422X