First detection and molecular characterisation of pseudocowpox virus in a cattle herd in Zambia

Table 2 Primers used in this study for the HRM assay and sequencing

Method	Primers’ ID	5ʹ → 3ʹ sequence	Amplicon size (bp)	Target
HRM	OPV-HRM-For	AGGACTAGCCGCGGTAACTTT	56	Orthopoxviruses
	OPV-HRM-Rev	ACAAGATAGAAGCGATGGATACTT	56	Orthopoxviruses
	CaPV-HRM-For	TCCTGGCATTTTAAGTAATGGT	100	Capripoxviruses
	CaPV-HRM-Rev	GTCAGATATAAACCCGGCAAGTG	100	Capripoxviruses
	PPV-HRM-For	TCGAAGATCTTGTCCAGGAAG	112	Parapoxviruses
	PPV-HRM-Rev	CCGAGAAGATCAACGAGGTC	112	Parapoxviruses
Sequencing	ORFV-B2Lf-For	GACCTTCCGCGCTTTAATTT	1210	Parapoxviruses
Sequencing	ORFV-B2Lf-Rev	CCCGCCTGCTAAAAGACT	1210	Parapoxviruses

ISSN: 1743-422X