Fig. 2

Heat inactivation of NiV in 90% human serum. Aliquots (200 μL) of NiV (3.0 × 10⁶ TCID₅₀/mL) were treated at 56 °C for the indicated times. NiV remaining in the samples were tested for the determination of infectious titers in Vero cells. The virus titers (TCID₅₀/mL) in the treated samples were determined as described in the Materials and Methods section. Heat treatments were performed in triplicate. The limit of detection (1.39 × 10² TCID₅₀/mL) is shown as a dashed line