Fig. 8
From: Characterization of accessory genes in coronavirus genomes
a Multiple alignment of ORFSa sequences, with X motifs in the reading frame of ORFS shown in blue and ORFSa in green. Start and stop codons of the overlapping genes are indicated by violet and red boxes, respectively. Bat-CoV (WIV16) sequence is from Genbank:KT444582. b Nucleotide and amino acid sequences of the novel ORF predicted to overlap the Spike protein in the genome of SARS-CoV. The nucleotide sequence segment (SARS-CoV:nt 22,732–22,926) encodes part (residues 414–478) of the RBD (residues 323–502) of the Spike protein (normal characters), while the reading frame + 1 encodes a potential overlapping ORF (italics), which we named Sa