Fig. 1

Confirmation of the expression of each membrane glycoprotein complex in 293FT cells transfected with each expression vector complex with an indirect immunofluorescence assay (IIFA) using antibodies to the tags fused with each membrane glycoprotein complex. The 293FT cells transfected with each designated plasmid or the combination of the plasmids were washed with phosphate buffered saline (PBS) (−), spotted on glass slides, and fixed and permeabilized with a methanol and acetone mixture. Glycoproteins that were expressed on each IIFA plate were described on the first line. Tag antibodies used as the primary antibodies are rabbit anti- HAT-IgG, mouse anti-FLAG, mouse myc-IgG, and rabbit anti-gO peptide antibody. The expression of Tag was distinguished by a secondary antibody (anti-rabbit IgG Dylight594 or anti-mouse IgG Dylight488). The tags fused with each gc were HAT (−gB, −gH, −gM, −pUL128), FLAG (−gL, −gN, −pUL130) and c-myc (−gO, −pUL131A)