Table 1 Oligoneucleotide primer sequences used for detection of dengue virus serogroup and identification of dengue virus serotypes using RT-PCR assays. The primer sequences and the expected PCR products are shown in the table as described by Lanciotti et al. 1992 26]
From: Association of genotype III of dengue virus serotype 3 with disease outbreak in Eastern Sudan, 2019
Primer | Sequence | PCR product |
|---|---|---|
D1 | 5′-TCAATATGCTGAAACGCGCGAGAAACCG-3′ | 511 bp |
D2 | 5′-TTGCACCAACAGTCAATGTCTTCAGGTTC-3′ | |
TS1 | 5′-CGTCTCAGTGATCCGGGGG-3′ | 482 (Dl and TS1) DENV-1 |
TS2 | 5′-CGCCACAAGGGCCATGAACAG-3′ | 119 (Dl and TS2) DENV-2 |
TS3 | 5′-TAACATCATCATGAGACAGAGC-3′ | 290 (Dl and TS3) DENV-3 |
TS4 | 5′-CTCTGTTGTCTTAAACAAGAGA-3′ | 392 (Dl and TS4) DENV-4 |