Fig. 3

Fluorescence profile of VSV-GFP (green channel), nuclei (blue channel), mitochondria (orange channel) and cell membrane morphology (black graph). Jurkat cells were analyzed by quantitative confocal microscopy. a Control Jurkat cells untreated with PPF and uninfected with VSV-GFP. b cells treated with PPF alone, with fluorescence profile of an intact cell (shown within the red box in the inset). c cells treated with PPF alone with fluorescence profile of an apoptotic cell (shown within the red box in the inset). d cells infected with VSV-GFP at a multiplicity of infection of 0.001 with fluorescence profile of a cell (shown within the red box in the inset). e and f cells were first treated with PPF, then infected with VSV-GFP. e shows the fluorescence profile of a large EV (shown within the red box in the inset), while f shows the fluorescence profile of a nearby infected cell