Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types

Gorgoglione, Bartolomeo; Ringiesn, Jeffery L.; Pham, Loc H.; Shepherd, Brian S.; Leaman, Douglas W.

doi:10.1186/s12985-020-01372-4

Virology Journal

Table 1 Oligonucleotides used for the construction of plasmids

From: Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types

Primer	Sequence (5′ → 3′)	Restriction Site	Primer Source	Sequence Source
IHNV-M N se	AGGAATTCATGACAGCGACACTCAGAG	EcoRI		HM461966 (AEH95651)
IHNV-M N as	AGGGTACCGTGGAATGAGTCGGAGTC	KpnI		HM461966 (AEH95651)
IHNV-M P se	AGGAATTCATGTCGATGGAGAAGGAG	EcoRI		HM461966 (AEH95652)
IHNV-M P as	AGGGTACCTTGACTTGCTTCATGCGC	KpnI		HM461966 (AEH95652)
IHNV-M M se	ACGAATTCATGTCTATTTTCAAGAGAGC	EcoRI	Ke et al., 2017 [35]	HM461966 (AEH95653)
IHNV-M M as	CTTGGTACCTTTTTCCTTCCCCCGCTTTTCGG	KpnI	Ke et al., 2017 [35]	HM461966 (AEH95653)
IHNV-M G se	AGAATTCGAGATGGACCATGATCACCAC	EcoRI		HM461966 (AEH95654)
IHNV-M G as	AGGTACCTTGGACCGGTTTGCCAGGTG	KpnI		HM461966 (AEH95654)
IHNV-M NV se	ACGAATTCATGGACCACCGCGACATAAACAC	EcoRI		HM461966 (AEH95655)
IHNV-M NV as	ACGGTACCTCTGGGATAAGCAAGAAAGTCTTC	KpnI		HM461966 (AEH95655)
VHSV-IVb N se	CAGAATTCATGGAAGGAGGAATC	EcoRI	Ke et al., 2017 [35]	KY359357 (ASZ84902)
VHSV-IVb N as	GTGGTACCATCAGAGTCCTCG	KpnI	Ke et al., 2017 [35]	KY359357 (ASZ84902)
VHSV-IVb P se	CAGAATTCATGACTGATATTGAGAT	EcoRI	Ke et al., 2017 [35]	KY359357 (ASZ84903)
VHSV-IVb P as	GTGGTACCCTCTAACTTGTCCA	KpnI	Ke et al., 2017 [35]	KY359357 (ASZ84903)
VHSV-IVb M se	ACGAATTCATGGCTCTATTCAAAAGAAAGCGCACCATCCTG	EcoRI	Ke et al., 2017 [35]	KY359357 (ASZ84904)
VHSV-IVb M as	ACGGTACCCCGGGGTCGGACAGAG	KpnI	Ke et al., 2017 [35]	KY359357 (ASZ84904)
VHSV-IVb G se	ACGAATTGATGGAATGGAATACTT	EcoRI	Ke et al., 2017 [35]	KY359357 (ASZ84905)
VHSV-IVb G as	GTGGTACCGACCATCTGGCT	KpnI	Ke et al., 2017 [35]	KY359357 (ASZ84905)
VHSV-IVb NV se	ACGAATTCATGACGACCCAGTCGGCAC	EcoRI	Ke et al., 2017 [35]	KY359357 (ASZ84906)
VHSV-IVb NV as	ACGGTACCTGGGGGAGATTCGGAGCCA	KpnI	Ke et al., 2017 [35]	KY359357 (ASZ84906)

The restriction enzyme recognition sites are shown in bold. Sequence source is provided for both the full viral genome and for specific genes (in parenthesis)

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ISSN: 1743-422X

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