Fig. 1

Real-time qPCR analyses of the specificity and sensitivity of primer pair designed for MDV detection. a Melting curve analyses; b Typical raw fluorescence data from a real-time qPCR assay for the standard MDV plasmid. NC is the negative control. c Reproducibility of the real-time qPCR assay. The Cq value is represented as mean ± standard deviation (SD). The standard regression line was generated based on 6 data sets