Fig. 4
Irradiation effect to cell invasion. Cytokeratin stained sections at × 50 magnification before (0 Gy) and after (4 Gy) irradiation in the HSC-3 (a), SiHa (b) and CaSki (c) are presented. After irradiation the invasion depth decreased from 1.25 μm to 0.66 μm in HSC-3 (p < 0.001), from 1.28 μm to 0.42 μm in SiHa (p < 0.001) and from 0.91 μm to 0.50 μm in CaSki (p < 0.01). Also, the invasion area was decreases in HSC-3 from 86.2 mm2 to 32.1 mm2, SiHa from 94.3 mm2 to 56.1 mm2 and CaSki from 74.9 mm2 to 39.0 mm2, but not with statistical significance. The differences in invasion depth (f) and invasion area (g) were measured and compared before (0 Gy) and after (4 Gy) irradiation. Statistically significant differences have been marked to figures as *p ≤ 0.001