Table 1 Comparison of purification performances between inverse transition cycling and affinity chromatography
Protein | Expression (mg/l) | Purity (%) | Recovery (%) | Yield (mg/l) | Purification time (h) |
|---|---|---|---|---|---|
ELP-Cap | 56.16 | 94.3 | 74.6 | 41.91 | 2.5 |
Cap-His | 49.37 | 90 | 60.77 | 30 | 23 |