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Fig. 1 | Virology Journal

Fig. 1

From: Generation and immunogenicity assessment of ELPylated virus-like particles of porcine circovirus type 2

Fig. 1

Expression of ELP-Cap and Cap-His fusion proteins in E. coli. a, b The schematic structures of pELP-Cap and pET-Cap vectors. The coding sequences for ELP, His-tag, Cap protein of PCV2b and the VN epitopes of PCV2a, PCV2d and PCV2e are indicated. The expression of fusion sequence was under the control of T7 promoter (PT7). c, d SDS-PAGE analysis of ELP-Cap and Cap-His fusion protein expression. The expression of two fusion proteins was induced for 24 h at 20 °C with 0.2 mM IPTG. After sonication treatment, the total cell lysates of recombinant E. coli before IPTG induction (1), after IPTG induction (2), supernatants (3) and pellets (4) of centrifuged cell lysates were analyzed by 12% SDS-PAGE. M indicates protein molecular mass marker. The small arrows indicate ELP-Cap and Cap-His fusion proteins

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Virology Journal

ISSN: 1743-422X