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Fig. 4 | Virology Journal

Fig. 4

From: Mitomycin, 5-fluorouracil, leflunomide, and mycophenolic acid directly promote hepatitis B virus replication and expression in vitro

Fig. 4

Replication of HBV genes in HepG2.2.15 cells stimulated by epirubicin, mitomycin, 5-fluorouracil, dexamethasone, leflunomide, and mycophenolic acid. HepG2.2.15 cells were exposed to increasing concentrations of cytotoxic chemotherapy drugs, epirubicin (a), mitomycin (b), and 5-fluorouracil (c), for 16 h, and increasing concentrations of immunosuppressants, dexamethasone (D), leflunomide (e), and mycophenolic acid (f), for 5 days. Intracellular capsid DNA was detected by molecular hybridization with the α-32P-labeled HBV DNA probe after NAGE (top). The number of extracellular HBV DNA copies was measured by qPCR (bottom), and the mean values from three isodose treatments, including SD, were statistically significant (p < 0.05)

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