Fig. 3
From: HSC70 is required for infectious bursal disease virus (IBDV) infection in DF-1 cells
IBDV infection in DF-1 cells blocked by anti-HSC70 antibody at different concentrations. a: IFA results showed DF-1 cells pretreated with the antibody to HSC70. The higher concentration of the antibody, the blocking effect better. Normal IgG: same isotype of IgG for control. Positive control: DF-1 cells infected with IBDV. b: The viral titers in DF-1 cells after pretreatment with the anti-HSC70 antibody at different concentrations. c: Western blot results of the protein expression level of VP2 in DF-1 cells treated with/without antibody to HSC70. Lane 1–3: the protein expression levels treated with anti-HSC70 antibody at concentration of 25 μg/mL, 12.5 μg/mL and 6.25 μg/mL respectively; lane 4: normal IgG as control; lane 5: DF-1 cells infected with IBDV as positive control; GAPDH: the internal reference control. The relative expression level of VP2 was quantified by normalizing GAPDH control. After data analysis, the values of VP2/GAPDH are 0, 0.537, 1.425, 3.329, and 3.345 from left to right