Fig. 2

Verification the interaction of PIAS4 and C protein through Co-immunoprecipitation. a Co-IP of PIAS4 with core. The indicated plasmids were transfected into HEK293T cell, and whole cell lysates obtained 48 hpt were immunoprecipitated with rabbit anti-Myc PAbs; Proteins were detected by western blot (WB) with mouse anti-Myc and anti-HA MAb. b Co-IP of BVDV core with endogenous PIAS4. BVDV-infected or mock-infected MDBK cells were used for IP with anti-C protein MAb and immunoblotted with the anti-C protein and anti-PIAS4 PAbs