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Fig. 1 | Virology Journal

Fig. 1

From: Disulfide isomerase ERp57 improves the stability and immunogenicity of H3N2 influenza virus hemagglutinin

Fig. 1

Overexpression and characterization of PDI proteins in 293 T cells. NC denotes the normal culture of 293 T cells; PDI and ERp57 denote overexpression of PDI and ERp57 proteins respectively; PDI/HA and ERp57/HA denote co-expression of H3 HA with ERp57 and PDI proteins respectively. a Western-blot analysis of PDIprotein expression under reducing condition. b Western-blot analysis of ERp57 protein expression under reducing condition. ImageJ was used to quantify PDIs. c Indirect immunofluorescence assay of PDI protein expression. d Indirect immunofluorescence assay of ERp57 protein expression. Cells nuclei were stained with DAPI (blue). PDI/ERp57 proteins were reacted with primary antibody and FITC/Cy3-conjugated secondary antibody (green/red). Scale bars indicate 25 μm

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Virology Journal

ISSN: 1743-422X