Fig. 5

The effect of truncated LT on its cognate promoter in MCC13 and HDF cells. (A) MCC13 cells were co-transfected with the luciferase reporter plasmid containing the early MKL-1 (MS-1, respectively) promoter, and either empty vector (EV), full-length LT, or truncated LT (tLT) encoded by the MKL-1 (MS-1, respectively) MCPyV variant; (b) as (a,) but luciferase reporter plasmids containing the late promoter of MKL-1 or MS-1 were used. Cells were also co-transfected with the reporter plasmid containing the early or late consensus promoter and empty vector or LT expression plasmid. (c) and (d) As (a) and (b), respectively, but transfections were performed in HDF cells. Luciferase activity was corrected for the protein concentration in each sample, with the activity in the presence of the empty vector arbitrarily set as 100%. Each bar represents the average of three independent + SD. Similar results were obtained in two additional experiment; *p < 0.05, **p < 0.01