Fig. 4

Aspergillipeptide D affects the expression and localization of viral Glycoprotein B. a the effect of Aspergillipeptide D on viral DNA synthesis. Vero cells infected with HSV-1 (MOI = 3) were incubated with or without Aspergillipeptide D (25 μM) for 15 h. The total DNA was extracted, and the expression level was detected. b the mRNA expression levels of viral immediate early gene UL54, early gene UL52 and late gene UL27 at 3, 6 and 9 h post-infection, respectively. Data are mean ± SD (n = 3). **p < 0.01 ***p < 0.001 versus HSV-1-treated group. cAspergillipeptide D reduced the protein level of HSV-1 gB. Vero cells were infected with HSV-1 for the indicated times and cell lysates were subjected to western blot assay for different HSV-1 proteins. d Densitometric analysis for gB western blot bands was shown. GAPDH served as a loading control. Data are mean ± SD (n = 3). **p < 0.01 versus HSV-1-treated group. e-f Immunofluorescence experiments demonstrated the location of gB in endoplasmic reticulum (e) and the Golgi apparatus (f) under the treatment of Aspergillipeptide D. Vero cells seeded in confocal dishes were treated with HSV-1(MOI = 3) or Aspergillipeptide D for 9 h. The cells were then stained with ER-Tracker Red (Red), Golgi-Tracker Red (red), DAPI (nucleus, blue) and anti-gB primary antibody (green). g Vero cells transfected with HA-labeled gB plasmid were infected with HSV-1 for 48 h in the presence or absence of Aspergillipeptide D. The virus titer was then measured