Fig. 2From: The 2b protein and C-terminal region of the 2a protein indispensably facilitate systemic movement of cucumber mosaic virus in radish with supplementary function by either the 3a or the coat proteinAccumulation of CMV in radish (cv. Tokinashi). a Detection of CMV in inoculated cotyledons at 10 dpi and b in the upper, non-inoculated leaves and roots at 21 dpi by tissue immunoblotting using antiserum against CMV-Y CP. Cotyledons of 7-day-old seedlings were mechanically inoculated with CMV-Y, CMV-D8, and their pseudorecombinants. Numbers at the top represent individual test plants, and numbers on the left indicate leaf positions from the top of the plants. M, mock-inoculated plants. c Results of RT-PCR targeting RNA3 using total RNAs from inoculated cotyledons (U, upper row) and upper, non-inoculated leaves (L, lower row) of radishBack to article page