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Fig. 2 | Virology Journal

Fig. 2

From: The 2b protein and C-terminal region of the 2a protein indispensably facilitate systemic movement of cucumber mosaic virus in radish with supplementary function by either the 3a or the coat protein

Fig. 2

Accumulation of CMV in radish (cv. Tokinashi). a Detection of CMV in inoculated cotyledons at 10 dpi and b in the upper, non-inoculated leaves and roots at 21 dpi by tissue immunoblotting using antiserum against CMV-Y CP. Cotyledons of 7-day-old seedlings were mechanically inoculated with CMV-Y, CMV-D8, and their pseudorecombinants. Numbers at the top represent individual test plants, and numbers on the left indicate leaf positions from the top of the plants. M, mock-inoculated plants. c Results of RT-PCR targeting RNA3 using total RNAs from inoculated cotyledons (U, upper row) and upper, non-inoculated leaves (L, lower row) of radish

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