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Fig. 4 | Virology Journal

Fig. 4

From: Characterization and a RT-RPA assay for rapid detection of Chilli Veinal mottle virus (ChiVMV) in tobacco

Fig. 4

Establishment of RT-RPA assay for ChiVMV detection. a Optimization of RPA reaction time. Lane M, Trans2K DNA marker; lanes 1–3, DNA products amplified by RPA reaction for 10 min, 20 min or 30 min, respectively. b Specificity analysis of RT-RPA. Lane M, Trans2K DNA marker; lane 1, ChiVMV-infected tobacco plants; lane 2, PVY-infected tobacco plants; lane 3, TSWV-infected tobacco plants; lane 4, TVBMV-infected tobacco plants; lane 5, distilled water control. c Sensitivity comparison of RT-RPA and RT-PCR. lane M, Trans2K DNA marker; lane 1–6, a series of dilutions of total RNA extracted from ChiVMV-infected tobacco plants with concentrations of 1 ng, 10 pg, 1 pg, 100 fg, 10 fg and 1 fg, respectively; lane 7, distilled water; lane 8, healthy tobacco plants

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Virology Journal

ISSN: 1743-422X