First report and genetic diversity of porcine bufavirus in China

Table 1 Primers used in this study

Fragment	Sequence of PCR primers	Position in genome^a	Length of PCR products^a
A^a	5′-TGACTATACTCTGGACATTAAC − 3′	1–22	1303 bp
A^a	5′-CATKATTGGTTGTCTGTGTTC − 3′	1283–1303	1303 bp
B^a	5′-CCATGCAATCATGTGCTGC −3′	916–934	1380 bp
B^a	5′-AGTTTGTTGTATTCCAAATCGT −3′	2274–2295	1380 bp
C^a	5′-TGGATACAACTATCTCGGACC −3′	2194–2214	1996 bp
C^a	5′-TATGTCTGGAAGGTTGTAGGT −3′	4169–4189	1996 bp
nPBuV-1F^b	5′-CCATGCAATCATGTGCTGC −3′	916–934	1380 bp
nPBuV-1R^b	5′-AGTTTGTTGTATTCCAAATCGT −3′	2274–2295	1380 bp
nPBuV-2F^b	5′-AAGAAGCAGGCAACCTAGG −3′	1110–1128	194 bp
nPBuV-2R^b	5′-CATKATTGGTTGTCTGTGTTC −3′	1283–1303	194 bp

^aPCR primers of the near-complete genome, position in the genome, and length of PCR products with respect to the porcine bufavirus strain 61 (accession no. KU867071) and genome
^bNested PCR primers of porcine bufavirus

ISSN: 1743-422X