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Table 1 Primers used in this study

From: First report and genetic diversity of porcine bufavirus in China

FragmentSequence of PCR primersPosition in genomeaLength of PCR productsa
Aa5′-TGACTATACTCTGGACATTAAC − 3′1–221303 bp
5′-CATKATTGGTTGTCTGTGTTC − 3′1283–1303
Ba5′-CCATGCAATCATGTGCTGC −3′916–9341380 bp
5′-AGTTTGTTGTATTCCAAATCGT −3′2274–2295
Ca5′-TGGATACAACTATCTCGGACC −3′2194–22141996 bp
5′-TATGTCTGGAAGGTTGTAGGT −3′4169–4189
nPBuV-1Fb5′-CCATGCAATCATGTGCTGC −3′916–9341380 bp
nPBuV-1Rb5′-AGTTTGTTGTATTCCAAATCGT −3′2274–2295
nPBuV-2Fb5′-AAGAAGCAGGCAACCTAGG −3′1110–1128194 bp
nPBuV-2Rb5′-CATKATTGGTTGTCTGTGTTC −3′1283–1303
  1. aPCR primers of the near-complete genome, position in the genome, and length of PCR products with respect to the porcine bufavirus strain 61 (accession no. KU867071) and genome
  2. bNested PCR primers of porcine bufavirus