Fig. 5
From: PCR-based reverse genetics strategy for bluetongue virus recovery
Growth curves of wild-type and recombinant viruses in BHK-21 cell. BHK-21 cells were infected with wtBTV1, rBTV1 and BTV1seg2, virus titers in the culture supernatant were determined at various time points post-infection. Results are presented as the mean viral titers of three independent experiments and error bars indicate the standard deviation. The viral titer of BTV1seg2 was lower than that of wtBTV1 and rBTV1 at 24 h and 48 h post infection, and then the titers were same at 72 h, 96 h and 120 h post infection. The titers of wtBTV1, rBTV1 and BTV1seg2 increased quickly within the 48 h post infection, and then the titer of each virus reach plateaus at 72 h post infection